yxaB [2017-10-19 18:15:00]

general stress protein, similar to pyruvyl transferase

Locus: BSU40030

Isoelectric point: 4.53

Molecular weight: 39.61 kDa

Protein length: 343 aa

Gene length: 1029 bp

Function: biofilm formation, survival of salt and ethanol stress

Product: putative exopolysaccaride synthase

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Other proteins required for biofilm formation
• Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
• Groups of genes → Poorly characterized/ putative enzymes

Gene

Coordinates: 4,110,949 → 4,111,980

The protein

Protein family

• polysaccharide pyruvyl transferase family (according to Swiss-Prot)

Paralogous protein(s)

• EpsI(32,4%)

Structure

• 5AX7 (from yeast, 30% identity)

Expression and Regulation

Operons

• Genes: yxaA-yxaB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigB: sigma factor, PubMed, in SigB regulon

  Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon

  Regulation

  • induced by stress (SigB) PubMed

• Genes: yxaB
  Description: PubMed

  Regulation

  • induced by stress (SigB) PubMed

Biological materials

Mutant

• MGNA-B681 (yxaB::erm), available at the NBRP B. subtilis, Japan
• BKE40030 (ΔyxaB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATCTGCGTCCTCCTTCT, downstream forward: _UP4_TAAACGAAAAAGACTGCCCT
• BKK40030 (ΔyxaB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATCTGCGTCCTCCTTCT, downstream forward: _UP4_TAAACGAAAAAGACTGCCCT

References

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